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名称 Proteomics in practice : a guide to successful experimental design
索引号 Q51/E20092.2
分类号 Q51
Q51(蛋白质)
Q5(生物化学)
Q(生物科学)
作者
出版社 Weinheim : Wiley-VCH ; Chichester : John Wiley [distributor], c2008.
ISBN
页数 xx, 482 p. : ill. (some col.) ; 25 cm.
价格
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简介
注解 Previous ed.: 2002. 附註 Includes bibliographical references and index. 內容 Preface -- Foreword -- Abbreviations, symbols, units -- Introduction -- Pt. I. Proteomics technology. Electrophoretic techniques ; Liquid chromatography techniques ; Mass spectrometry ; Functional proteomics: studies of protein-protein interactions -- Pt. II. Practical manual of proteome analysis. Equipment, consumables, reagents ; Step 1: Sample preparation ; Step 2: Fluorescence different gel electrophoresis ; Step 3: Isoelectric focusing ; Step 4: SDS polyacrylamide gel electrophoresis ; Step 5: Scanning of gels containing pre-labeled proteins ; Step 6: Staining of gels ; Step 7: Image analysis and evaluation of DIGE gels ; Step 8: Spot excision ; Step 9: Sample destaining ; Step 10: Protein digestion ; Step 11: Microscale desalting and concentrating of sample ; Step 12: Chemical derivatization of the peptide digest ; Step 13: MS analysis ; Step 14: Calibration of MALDI-ToF MS ; Step 15: Preparing for a database search -- Pt. III. Trouble shooting -- Glossary of terms.